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After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

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Chan HL, Chan CK, Hui AJ, Chan S, Poordad F, Chang TT, Mathurin P, Flaherty JF, Lin L, Corsa A, Gaggar A, Subramanian GM, McHutchison JG, Lau G, Lee S, Gane EJ

Furthermore, there is emerging evidence for the intergenerational effects of maternal B-vitamin status during pregnancy on the offspring development (Reference Finkelstein, Fothergill and Venkatramanan89,Reference McNulty, Rollins and Cassidy96,Reference Caffrey, McNulty and Rollins97)

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