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1b,c and 3k, pancreata were weighed, then mechanically dissociated, followed by digestion in 5 ml of HBSS containing collagenase I (375 U ml 1 ), DNase I (0.15 mg ml 1 ) and Soybean Trypsin inhibitor (Sigma, 0.05 mg ml 1 ) for 30 min at 37 C on a shaker (220 rpm), followed by dissociation with a syringe and needle, filtration through a 70-m strainer to exclude Langerhans islets and red blood cell lysis

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Anterior or posterior to the first rib is the hyperechoic pleura, with lung tissue deep to it

this is an important step for its anti-ferroptosis activity

doi: 10.1007/s00011-023-01774-4 42 ChalmersJDShteinbergMMallMAO'DonnellAEWatzHGuptaAet al