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Neurology 57 , 159160 (2001)

With the purpose of hinder the employ of exogenous and potentially morbigenous albumin and to gain greater specific drug preparation, a MTX albumin-linking prodrug EMc-D-Alaphe-Lys-Lys (gamma-MTX) OH (EMC = 6-maleimide hexpropionic acid) has been developed by Kratz et al

The reported effects were not dose-dependent

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HEK293T cells in a T-75 flask (~7080% confluency) were transfected with AMS3a E1E2 plasmid using PEI MAX