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All cell lines were tested for Mycoplasma contamination (Mycoalert Mycoplasma Detection Kit (Lonza)

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As shown in Figure 2A, after continuous scanning using a depletion laser, fluorescence signals of the commercial dye, BODIPY 493/503, were quickly quenched, whereas that of DTPA-BT-M remained unchanged, indicating its high photobleaching resistance in STED imaging

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