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In general, gene mutations with large biological effects (i.e., optic neuropathy) are rare whereas gene variants associated with smaller effects are common (12) (see below)

10.1371/journal.pone.0101815 PLoS ONE 66 Rodriguez-GaztelumendiA.AlvehusM.AnderssonT.JacobssonS

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It's caused by cold bacteriostatic water, rapid plunger pressure, or incomplete reconstitution that leaves undissolved particles in solution

The funnel plot showed asymmetry between studies on both sides, suggesting the potential presence of publication bias (Figure 8)