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Briefly, total RNA (1 g) was denatured at 65 C for 5 min together with random hexadeoxynucleotide primers (2.5 M)

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12 , 13 Individuals with vitamin B12 deficiency may be at greater risk of patient safety incidents in primary care as they often describe suboptimal communication, lack of dignity and respect, and feelings that healthcare professionals lack the knowledge, skills, and attitudes to adequately treat their condition