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Mast Cell Activation and Histamine Release Certain peptides, particularly cationic (positively charged) or biologically active signaling peptides, have been shown in research settings to stimulate mast cell degranulation

To Eliminate non-specific adsorbents caused by hydrophobic adsorption: Wash with 3-4 column bed volumes of 70% ethanol or 2 column bed volumes of 1% Triton X-100, followed immediately by 5 column bed volumes of PBS, pH 7.4.

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platensis cells were high-pressure frozen using a Leica EM PACT2 instrument (Leica Microsystems, Buffalo Grove, IL), cryo-substituted for 80 h at 87 C in acetone containing 2% osmium tetroxide, and, after slowly warming to RT, embedded in Spurrs resin

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