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Expression of recombinant Ec Lgt expression was induced by adding isopropyl -D-1-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM

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Lowering oxygen levels to 5%, 1% and 0.1% for 6 h before re-oxygenation to ambient levels confirmed fast and reversible NAD reduction in Arabidopsis leaf tissue at 1 and 0.1% oxygen ( Figure 4FJ and Figure S9 )

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