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The availability of this reagent, which allows organoids to grow in collagen-only hydrogels, may avoid the need for BME or Matrigel

Therefore, we included a total of 87 articles in this study (eReference), and a flowchart is presented in eFig

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SAM is an all-purpose methyl group donor that participates as a co-factor in a variety of physiological and cellular processes, including lipid homeostasis

The parasacral area is delineated by the ventral aponeurosis of the piriformis muscle dorsally, by the pelvic aponeurosis medially, and by the aponeurosis of the obturator internis muscle laterally