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Such as under controlled experimental conditions, e-cigarette aerosol or nicotine exposure consistently disrupts tight junction organization, leading to reduced expression of ZO-1 and claudin-3, increased oxidative stress, and upregulation of pro-inflammatory adhesion molecules

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The sample was further centrifuged at 17,400 g for 30 min at 4 C and the supernatant was used as the cytosolic fraction

The system LOD is sufficiently low to detect nicotine and aldehydes emitted from most e-cigarette devices (Khlystov and Samburova, 2016

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Prolactin is the primary hormone involved in milk production, so reduced prolactin levels cause a reduced milk supply