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Description

Neurology 95 , e3012e3025 (2020)

Human CBS was cloned into pcDNA5-FRT encoding for a C-terminal Flag-mKO2-tag for the mammalian expression vectors

PI, oxidized PI (PI Ox ), PS, oxidized PS (PS Ox ), lysophosphatidylinositol (LPI) and lysophosphatidylserine (LPS) were detected in the 2:1 extract, by negative-ion mode FTMS as deprotonated ions by scanning m/z = 4001,100 Da, at R m/z = 200 = 280,000 with lock mass activated at a common background ( m/z = 529.46262) for 30 s