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The cells were then washed three times with PBS, drained and mounted

Physiol Plant 119:1929 Ding S, Lu Q, Zhang Y, Yang Z, Wen X, Zhang L, Lu C (2009) Enhanced sensitivity to oxidative stress in transgenic tobacco plants with decreased glutathione reductase activity leads to a decrease in ascorbate pool and ascorbate redox state

Eur J Hum Genet 30 , 889898 (2022)

Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36

The main advantage of using either chaotropic salt of a change in ionic strength is that this usually leads to gentle elution of the target in an active form [8]

[35] In rodent pre-clinical studies, urinary and serum alpha GST have been shown to be sensitive and specific indicators of renal proximal tubular and hepatocyte necrosis respectively