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(2018) 62(1):10.1002/mnfr.201700447

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A solution of aqueous NaOH was added and heated for 4 h at 80

The freely floating tissue sections containing corpus callosum lining around lateral ventricle and caudate nucleus were selected and treated with 1% Triton X-100 in PBS at 4 C overnight and incubated with primary antibody at a dilution of 1:200 in PBS containing 0.1% Triton X-100 and 1% HS at 4 C overnight

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Healthy individuals usually absorb iron supplements (e.g., iron salts or polysaccharide-iron complex) adequately from the GI tract, but some patients may benefit from receiving supplemental ascorbic acid with each oral iron dose