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Protein was further purified by size-exclusion chromatography with a Hiload 16/60 Superdex 200 pg column under an isocratic condition (20 mM Tris-HCl pH 7.5 and 150 ml NaCl), and its purity was estimated by 10% SDS-PAGE

Bryn V, Halvorsen B, Ueland T et al (2015) Brain derived neurotrophic factor (BDNF) and autism spectrum disorders (ASD) in childhood

That structure is stable in lyophilised powder form but begins hydrolyzing the moment it contacts water, even at low temperatures

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( AD ) Representative fluorescence measurement of RBC (purple) and iRBC (green) within a defined region of interest (ROI)

doi: 10.3390/ijms22083872 91 LehmannM