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Samples (10 g per lane) were resolved on pre-casted gradient SDS-polyacrylamide gel electrophoresis gels (Biorad) under reducing conditions and electrotransferred to nitrocellulose membranes

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However, the essential oil contains coumarins, which can cause phototoxicity in humans and have been linked to tumor formation on the skin and foregut of mice

Ann Transl Med 9:355 EI Makawy AI, Abdel-Aziem SH, Mohammed SE, Ibrahim FM, Abd EL-kader HA, Sharaf HA et al (2024) Exploration of tumor growth regression of quinoa and chia oil nanocapsules via the control of PIK3CA and MYC expression, anti-inflammation and cell proliferation inhibition, and their hepatorenal safety in rat breast cancer model

Lpez-Otn C, Blasco MA, Partridge L et al (2023) Hallmarks of aging: an expanding universe