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Neuromasts were masked using a line tool and mean fluorescence was measured for an entire neuromast using ImageJ version 1.48 (National Institutes of Health, Bethesda, MD), mean background fluorescence was then subtracted out to yield net mean fluorescence for each neuromast

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Cycling Alternating periods of peptide use with rest periods to prevent receptor desensitization and allow physiological recovery

Peptides are short chains of amino acids, the "building blocks" of more complex proteins

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