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Case Studies Case 1: TEV Won't Cut Protein: 45 kDa enzyme Construct: His6-TEV site-Target Problem: No cleavage after 24 hours with excess TEV Diagnosis: Crystal structure of homolog showed N-terminus buried in a groove TEV site was inaccessible to protease Solution: Redesigned: His6-(GGGGS)-TEV site-(GGGGS)-Target Cleavage now complete in 4 hours Case 2: Thrombin Destroys the Protein Protein: 30 kDa signaling domain Construct: His6-Thrombin site-Target Problem: Multiple bands after cleavage

Its sad to say, but thats what I see

The effect of a topical antioxidant formulation including N-acetyl carnosine on canine cataract: a preliminary study

JCI Insight 5(20) 2020 PMC7605543 Ginsberg C, Zelnick LR, Block GA, Chertow GM, Chonchol M, Hoofnagle A, Kestenbaum B, de Boer IH

What Are the 7 More Dangerous Medicines to Mix?Some drug interactions can have serious side effects, while others may be lethal

Markers of lipid peroxidation and tocopherol levels in the blood and neoplastic tissue of dogs with malignant mammary gland tumors