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In the beginning, 6-OHDA was found to lessen noradrenaline concentrations in the brains of mice (Porter et al., 1965)

And so then we is just gonna remain how that plays out

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A six-minute gradient elution program was optimized as follows: Initial conditions (10% A, 90% B) transitioned linearly to 50% A at 1.5 min, rapidly increased to 95% A at 1.6 min (held until 4.5 min), and returned to initial conditions at 4.6 min for re-equilibration