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Reactions were incubated at room temperature for 20 min prior to reading at 490 nm using an Infinite F200 Pro plate reader (Tecan)

This therapy increased survival and reduced tumor size (56)

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In an in vitro study, Laiguillon (2015) used diverse methods such as (14C)-2-deoxyglucose to assess glucose uptake, quantitative RT-PCR, and ELISA/EIA to observe the expression and release of pro-inflammatory mediators, and ROS and nitric oxide (NO) production were measured

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The implications of this are manifold, as HP is a reversible disease in the early stages, reduced exposure to the inciting antigen as well as polluted air could possibly prevent disease progression