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Take 10 to 20 seconds if needed

10.1016/j.brainres.2014.12.026 231

Peak sodium nitrite blood concentrations ranged from 3.9 mg/l (56.52 mol/l), with an administered dose of 2.5 mg/kg, to 32.5 mg/l (471.01 mol/l) with an administered dose of 30 mg/kg, and corresponded to peak maternal methaemoglobin levels of 3.4% (2.5 mg/kg dose) to 60.2% (30 mg/kg dose) (Gruener et al., 1973)

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A closed system does not allow excess pressure to make it back out into the municipal supply

Keep the vial in its original box or a dark container to protect it from light, as prolonged light exposure can also contribute to degradation