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TRH binding was initially observed in the plasma membrane of the anterior pituitary extracted from cattle (191), and the receptor was later identified as a GPCR in mice (192)

The sample preparation is based on protein precipitation and derivatisation followed by HPLC determination with fluorescence detection

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(35880813) If symptoms are markedly improved but not absent despite a moderate/high dose of buprenorphine, consider the addition of adjunctive therapies