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The distribution of these monocyte subsets in peripheral blood is: ~ 85% classical monocytes (CD14+CD16-), ~5% intermediate monocytes (CD14+CD16dim) and ~7% non-classical monocytes (CD14dimCD16+) ( Figure 1 2.2 Monocyte function & erythropoiesis Each monocyte subset has its own gene expression and DNA methylation profiles, with varying phagocytic activity and chemokine receptor expression patterns ( Under steady state, classical monocytes are retained in the BM and spleen and are only dispatched to inflamed tissues when triggered by chemokines

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Bouret, S., Levin, B

Front Immunol (2020) 11:603831

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The manual should always be readily available to all testing personnel