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Intrastromal surgery preserved cellular layers, avoiding long-term deficits of nerves, epithelial cells and keratocytes seen in standard surgery 40,41

Acknowledgments The authors would like to thank the barn staff at IRTA-Facilities, for taking care of the animal management and its support during sampling

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This review argues that future research should integrate molecular, circuit, and behavioral perspectives, with emphasis on cell-typespecific receptor dynamics, adaptive plasticity under different nicotine exposure conditions, and individual genetic risk factors