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Cells were plated at 10 6 cells/well and cultured overnight (5% CO 2 , 37 C), then stimulated for 24 h at either a low (0.25 mM) or high (4.25 mM) concentration of the 33-mer peptide, or its Clc-derived fragments (9-mer, 7-mer and 3-mer

SS-31 is strictly for laboratory research only and is not approved for therapeutic or veterinary application

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It's a primary regulator of actin, a protein that is fundamental to cell structure, movement, and division

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No interaction is expected mechanistically