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Vina JR, Palacin M, Puertes IR, Hernandez R, Vina J (1989) Role of the gamma-glutamyl cycle in the regulation of amino acid translocation

NAC can be administered intravenously or orally, as an aerosol spray, or in liquid or powder form

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For validation of the assay method, the kinetic parameters such as the apparent Michaelis-Mente constants and maximum rates of conjugate formation as well as the specific activity of rat hepatic glutathione transferase were determined

Pulsechase labelling and immunoprecipitation Exponential phase cells were suspended in 10ml SD medium lacking methionine (6 10 7 cells/ml), pulselabelled with 500 Ci of [ 35 S] methionine (NEN, PerkinElmer) for 7 min and chased with excess cold methionine

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