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coli variants with the Thr35 Asp and Thr35 Asp/Asn37 Ser/Arg57 His mutations in the active site were compact and have pronounced secondary structure, they did not possess rigid 3D structure and were 10 times more susceptible to limited trypsinolysis in comparison with the wild type protein, emphasizing low proteolytic stability of these intrinsically disordered molten globular mutants (Leontiev et al., 1993)

Always remember that best practice to inject the water into the peptide vial is along the glass wall of the container to minimize foam and disturbance to the lyophilized peptide

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thetaiotaomicron (100 l overnight culture) in 10 ml of pre-reduced low-glucose Dulbeccos Modified Eagles medium (Gibco Life Technologies, Grand Island, NY, USA) supplemented with cyanocobalamin at 0 ppm, 0.01 ppm, and 15 ppm, which was subsequently incubated for 6 h