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Glutamine and/or glutamate serve as key substrates, compensating for metabolic alterations by enhancing pathways involved in lipid and glutathione synthesis

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Indeed, cooking, especially boiling or frying, can lower glutathione levels

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Avidin beads (avidin-agarose from egg white, 1.1 mg ml 1 in aqeuous glycerol suspension, Sigma-Aldrich) were carefully resuspended by inversion on an Eppendorf tube wheel at 4 C and transferred to an Eppendorf tube