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doi: 10.1016/j.biopha.2021.112164 85

Samples are then added into these wells and bind to the immobilized (capture) antibody

Protein purification and modification validation To further validate the S-glutathionylated cystine modification results from proteomic analyses, targeted candidate proteins were cloned into the vector pET21a with a C-terminal 6 His-tag and expressed in the E