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Relative gene expression was calculated using the comparative Ct method (2 Ct ) 41

10.1038/jid.2008.197 66

Only two forms of B12 are active as a coenzyme in the body: Methylcobalamin and adenosylcobalamin are the active coenzyme forms of B12

As expected, the African human SFB 16S rRNA gene sequences fell within the SFB clade when using as an outgroup four Clostridium species representing different Clostridium clades (Fig