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Spectra were acquired using a stimulated echo acquisition mode (STEAM, [39]) pulse sequence (TE/TM = 11/32 ms, TR = 5 s, number of transients = 64) with variable power radiofrequency pulses with optimised relaxation delays (VAPOR, [40]) water suppression and outer volume saturation [41]

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In a similar study, Attia [82] showed that the supply of betaine alone (1000 mg/kg), or with vitamin C (200 mg/kg) or vitamin E (150 mg/kg) to chronic HS roosters (38 1 C

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