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The following primers to amplify the fragment were designed using the homologous recombination method: GRFT-SmaI(F): 5-GtctcgaggaccggtcccgggATGAAGGCTTTCACTCTTGCCT-3 and GRFT-PmlI(R): 5-ctggtcaccaattcacacgtgATACTGCTCGTAATAGATATCAAGGGA-3

The musculoskeletal system is a comprehensive and interconnected system, and diabetic patients with muscle wasting syndrome are more prone to developing osteoporosis, and lower muscle mass and strength, along with higher fat content, can impair bone quality (Herrmann et al., 2020)

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Kidney disease is associated with in-hospital death of patients with COVID-19

Given the limited treatment options for these liver injuries, understanding the mechanisms and modes of cell death is crucial for identifying novel therapeutic targets

In some cases, the mechanism of injury is unknown, e.g