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Circulating CAR T cells were identified using anti-human CD45 (BD Biosciences) and CountBright beads (Thermo Fisher)

Liver PSAT1 is required for SAAR-induced improvements in systemic glucose homeostasis To assess whether hepatic serine synthesis is essential for the metabolic benefits of dietary SAAR on glucose control, we studied liver-specific PSAT1 KO mice and WT littermates fed a HF-CTRL or -SAAR diet (Fig