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The AFBO is highly unstable, and it reacts with cellular nucleophiles and can induce mutations by alkylating DNA, principally at the N 7 position of guanine forming the 8,9-dihydro- 8-(N 7 -guanyl)-9-hydroxy-AFB 1

The Caco-2 cell line was cultured in Minimum Essential Medium (MEM) (Gibco, NY, USA) supplemented with 20% FBS at 5% CO 2 at 37 C