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With age, expression of antioxidant enzymes and NADPH production in GCs decreases, resulting in a lower capacity to neutralize ROS

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Microscopic studies were carried out using a Zeiss Axiowert 200 M microscope (Carl Zeiss, Jena, Germany) equipped with a high-resolution digital camera (Axiocam HR, HQ CCD, Carl Zeiss, Jena, Germany) and filter set 10 (exc.: 535585 nm, em.: 600655 nm) for FDA, filter set 9 (exc.: 450490 nm, em.: 515 nm) for DHE, or filter set 20HE (exc.: 546/12, em.: 607/80) for Amplex Red was used