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The following concentrations were used: 2mM Malate + 5mM Pyruvate (LEAK respiration), 5mM ADP (OXPHOS capacity), 10M cytochrome c (integrity of outer mt-membrane), 10mM glutamate (OXPHOS capacity), 10mM succinate (OXPHOS capacity), 0.5M FCCP (ETS capacity, ETSCI&CII), 0.5M rotenone (ETS capacity, ETSCII), 2.5M antimycin A (residual oxygen consumption, ROX) were used in with high-resolution Oxygraph-2k (OROBOROS Instruments)

After the target protein is modified, UFM1 can be cleaved off by UFSP1 or UFSP2, releasing the UFM1 molecule for reuse in new biological cycles (99, 100)

Each cell has its own energy-producing department called a mitochondria

1 This impacts how our cells function

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Increased levels of energy, more fuel to keep the body working, and decrease in levels of fatigue, are a few of the key benefits youll notice from the onset