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Before fixation, HCK cells were stimulated for 24 h with 5 ng/ml human recombinant transforming growth factor beta 1 (TGF1, ab50036, Abcam, Cambrigde, United Kingdom), 10 mol/l capsazepine (CPZ), 1 mmol/l L-carnitine, 5 ng/ml TGF1 plus 10 mol/l CPZ, whereas 5 ng/ml TGF1 plus 1 mmol/l L-carnitine with Dulbeccos Modified Eagle Medium (DMEM) served as a control

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An enzyme-linked immunosorbent assay (ELISA) revealed that the binding affinities of scFv-GFPA36 and scFv-T7-A36 to glutathione S-transferase (GST)-Syt I-C2A were significantly lower than that of STAND-A36 (Fig