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After a series of carefully controlled washing steps, the bound insulin is detected by adding a substrate that produces a detectable signal, typically a colored product, in proportion to the amount of insulin present

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doi: 10.1002/fsn3.2145 29

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However, whether these molecular pathways contribute to the reduction of oxidative stress by NAC in LE-induced PCOS mouse ovaries in our study, or if other molecular pathways are involved, remains to be investigated