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However, when this strain was treated with BSO, an inhibitor of GSH synthesis, its ability to counteract the oxidative stress dropped, demonstrating that an active GSH synthesis was the cause of CO23FLC viability in the oxidative environment

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[25] Shen F-C, Weng S-W, Tsao C-F, Lin H-Y, Chang C-S, Lin C-Y, et al

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substrate nicotinamide at 100 M, methyl donor S-adenosyl-L-methionine (SAM) at 5 M, and S-adenosyl methionine (SAH) at 5 M concentration) samples were also run individually in the reaction buffer [1 mM Tris [pH 8.6], 1 mM DTT, 10% trichloroacetic acid, 4% methanol, and water] to identify elution time and define substrate, co-factor, and product peaks

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