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doi: 10.1155/2021/9991001 96 ZhaoYWangQZhuJCaiJFengXSongQet al

For reference range verification, the samples were frozen within 30-60 minutes from collection

Before a typical experiment, 135,000140,000 cells were seeded in a 6-cm dish and cultured at a density of 37,000 cells per 12-well plate and grown for 4446 h before transfection