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Interestingly, ablating IGF-I in the liver does not affect PTH-induced anabolism in bone (85)

Furthermore, a review of commercial supplements revealed that 400 mg is the maximum single dose commonly available, making 406 mg a reasonable and practical choice for our study

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To verify the conjecture, we transfected the glucose kinase and protein kinase -dead mutant (S340A/S788A) as well as D209A/D657A mutant, which retains protein kinase function but is glucose kinase-deficient, into HK2-KO MEFs 52,53

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