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Acid sphingomyelinase mediates ferroptosis induced by high glucose via autophagic degradation of GPX4 in type 2 diabetic osteoporosis

Studies have demonstrated that enhancement of ROS and lipid peroxidation is one of the culprits in the elevation of -SMA and COL1A1, indicative of myofibroblast formation

The protein free supernatant was collected and mixed immediately with 0.5 mL of NADPH (4 mg of reduced form was dissolved in 100 mL of 0.5% NaHCO 3 ), 0.5 mL of glutathione reductase (6 units/mL in 0.1 M phosphate buffer, pH 7.0) and 1 mL of 0.6 M DTNB (prepared in 0.2 M phosphate buffer of pH 8)

Srensen et al., 2005)

When we administered both compounds to them, we saw a reduction in the food and food intake for the diabetic rats, he explains

Separovich, R