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Afterwards, the mixtures were centrifuged at 2,500 rpm at 4C for 10 min and 20 l of the supernatant that contained the -GC product was added to a half-area black 96-well plate (Corning, 3904)

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PLGA nanoparticles, for instance, have shown promise in enhancing the immunogenicity of tuberculosis vaccines in multiple animal systems [200]

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Supplementary Table S1), the level of CD14 antigen was monitored in all samples during mass spectrometry analysis to follow the technical quality of analyses (Figure 1D)

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