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In dogs, a much larger study involving 51 dogs with hypocobalaminemia has been published (Toresson et al, JVIM 2016)

Cell culture and analysis JEG3 human choriocarcinoma cells JEG3 cells (ATCCHTB36 TM ) were cultured in six-well plates or coverslips in 20% O 2 at 37C in Eagle's Minimum Essential Medium (EMEM) media (ATCC, 30-2003) containing FBS and penicillinstreptomycin (Visent Inc)

Autophosphorylation assay and mass spectrometry Intact protein mass spectrometry was used to monitor transphosphorylation of TcPINK1-D337N by 15 N-TcPINK1-WT, as described previously 10

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The main signaling pathways downstream of the IGF-1 receptor are Ras/MAPK and PI3K/Akt, while these two pathways are responsible for the glucose and lipid metabolism in the liver ( Crosstalk Between Circadian Clock System and GH/IGF-1 Axis Regulatory Effect of the GH/IGF-1 Axis on the Circadian Clock System There are very few reports to identify the regulatory effect of the GH/IGF-1 axis on the circadian clock system

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