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Description

Ciprofloxacin powder (MilliporeSigma 17850) was dissolved in dilute acid (0.1 M HCl)

RNA concentrations were measured using Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE)

250-500

SDI, sociodemographic index

These samples assisted in identifying baseline FOXO4 expression patterns and age-related changes in Leydig cells, with all procedures strictly adhering to ethical guidelines and informed consent protocols [2]

The pharmacokinetic and toxicity properties of the lead compounds (CB-27, CB-41, CB-50, CB-53, CB-58, CB-59, and CB-61) were compared to CQ (Supplementary Table S6)