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Real-time PCR Total RNA, from either frozen liver tissues or cultured cells, was isolated by phenol-chloroform extraction

Glutathione is present in most cells of the body with concentrations highest in the liver

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The GSH transport between the various cell compartments is vital to buffer reactive oxygen species (ROS) and facilitate redox signaling in order to control cell growth, development and defense, as well as regulate cell proliferation

78 A statistical analysis that included nine randomized controlled trials, involving a total of 591 women with PCOS, also reported finding omega-3 fatty acid supplementation improved insulin sensitivity, lowered total cholesterol and triglyceride levels, and raised levels of adiponectin (a signaling peptide primarily produced by fat tissue that improves insulin sensitivity and has anti-inflammatory properties)