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For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions

Urinary GHB concentrations in people suffering from diabetes (N = 20) ranged from 0.17-3.03 mg/L compared with 0.16-2.14 mg/L in healthy control individuals (N = 30)

Therefore, in most cases, it is recommended to continue the pregnancy

103 Interestingly, sleep deprivation has been shown to reduce synthesis of serotonin and melatonin from tryptophan, instead favoring tryptophan breakdown into compounds that may have toxic effects in the brain

10.1016/j.neubiorev.2017.03.007 61 LeeJ.HyunD