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G.et al (2018)

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We transfected 293T cells with 5 g of plasmid (PGMLV-SC5:psPAX2:pMD2.G=4:3:1) and 7.5 L of Lipofectamine 3000

The primary inoculum (1 mL) was transferred into 140 mL of fresh LB medium in a 500 mL flask and cultured for 8 h to obtain the secondary inoculum, which was inoculated directly into the bioreactor

Bioavailability and bioavailable forms of collagen after oral administration to rats