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The cells were lysed on ice for 30 min in IP buffer (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.5% NP40, 10% glycerol) with protease inhibitor mixture, and the lysates were cleared by centrifugation

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Rosales-Corral S, Acua-Castroviejo D, Coto-Montes A et al (2012) Alzheimers disease: pathological mechanisms and the beneficial role of melatonin

Why the Symptoms Are So Often Missed There are a few predictable reasons B12 deficiency goes undiagnosed in post-weight-loss patients

36:11051112 Ahmed A , Kothari MJ : Recovery of neurologic dysfunction with early intervention of vitamin B12