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(Glutathione) 3 (Cystein) (Glycine) (Glutamine) 1 2 Reduced glutathione glutathione sulfhydryl (GSH) (thiol-SH) active Hydrogen peroxide (H2O2) GSH peroxidase Glutathione-S-transferases (GSTs) 2 Oxidized glutathione glutathione disulfide (GSSG) reduced glutathione 2 (S-S) GSSG GSH GSH reductase riboflavin GSSG GSH oxidative stress (tyrosine) (tyrosinase) 2 (eumelanin) (pheomelanin) 3 4 3 5 (melanin) (pheomelanin) (eumelanin) recycling active form 6 glutathione peroxidase Hydrogen peroxide (H 2 O 2 ) recycling coenzyme Q 10 Glutathione-S-transferases (GSTs) conjugate Acetaldehyde oxidation pathway neutrophils 7 8 9 L-Cysteine, L-Glutamine, L-Glycine 3 Alpha Lipoic Acid Glutathione alpha lipoic acid Coenzyme Q10 10 , , , Resveratrol natural antioxidants 11 N-acetylcysteine (NAC) L-cysteine GSH 12 Coenzyme Q10 oxidative stress antioxidant enzyme glutathione peroxidase 13 Milk Thistle Extract glutathione peroxides 14 Selenium cofactor glutathione peroxidase 15

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Nature 441 , 4652 (2006)

Expression and purification of GST fusion proteins