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Copper, oxidative stress, and human health

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The filtered gene-cell matrix were processed using the Seurat package (v.4.3.0) 70 , which included normalization using the NormalizeData function to obtain normalized UMI (unique molecular identifier) count data, scaling, and centering the data using ScaleData , finding the most significant principal components (PCA) using RunPCA and using the ElbowPlot to determine the number of principal components used for clustering

Given that EtOH-induced mitochondrial dysfunction and ER stress are intimately involved in the pathogenesis of ALD, future studies to examine these pathways under the current experimental setting will provide important mechanistic information underlying the protection seen in GCLM KO mice

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